The purpose of the present investigation was to assess and compare the Attitude of urban and rural population towards yoga. In the present study, the subjects for data collection were collected from urban (city) and rural (village) area in Allahabad, Uttar- Pradesh. Out of available population, age ranged from 35 to 55 years, the sample consisted of four hundred and fifty (N=450) population which further divided into two groups (n=225) urban peoples and (n=225) rural peoples. To measure the level of Attitude of urban and rural population towards yoga, psychological questionnaire “Kaivalyadhama yoga attitude scale” which was developed by Kocher (1976) and further standardized by Bhogal and Bera (2005) was applied. To compare the attitude of the urban and rural population towards yoga, 2×7 Factorial ANOVA test was applied to find out the significant differences between urban and rural population.