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ESTABLISHMENT OF CALLUS CULTURES AND THEIR PHYSICO- BIOCHEMICAL ANALYSIS IN BARLERIA LUPULINA LINDL.
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Author(s):
SUSHIL KUMAR
Vol - 9, Issue- 11 ,
Page(s) : 244 - 252
(2018 )
DOI : https://doi.org/10.32804/IRJMSH
Abstract
Barleria lupulina Lindl. (Family-Acanthaceae) is a small shrub, commonly called Sornomukhi. It is widely distributed in South East Asia and Eastern Africa. Tribals use different parts of Barleria lupulina to treat a number of ailments and disorders. This plant is popular in Thai folk medicine because it is used externally as an anti-inflammatory agent against different viral lesions caused by varicella zoster, herpes simplex and herpes zoster viruses. It is also used to alleviate inflammation due to snake and insect bites and also has a diuretic effect and anti-amoebic properties. Nine iridoid glucosides have been isolated from different parts. Compounds like barlerin, acetylbarlerin shanzhiside methyl ester, acetylshanzhiside methyl ester, ipolamiidoside and iridoid glucosides have beenisolated and identified from the leaves of Barleria lupulina Lindl. Young leaves, petioles and stems up to 5th node from apex of Barleria lupulina grown in glass house under controlled conditions were used as explants. The explants were inoculated for establishing callus culture. For culture initiation MS medium (Murasige and Skoog, 1962) with 30 g/l sucrose and 8.0 g/l agar, supplemented with different growth regulators was used for culture establishment. Auxins viz. 2,4-D, NAA, IBA, IAA and 2,4,5-T and cytokinins viz. Kn and BAP were used individually or in combination. High callus growth response with compact green callus was recorded on MS + 1.0mg/l NAA + 0.5mg/l BAP as compared to other growth regulator combinations in Barleria lupulina. Barleria lupulina calli subcultured on 1.0mg/l NAA with 1.0 and 2.0mg/l BAP resulted in higher protein, nitrogen and total sugar content after 4 weeks, though with lesser callus growth response than on NAA+0.5 mg/l BAP.
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