This study was conducted on different physiological variables among University level Basketball and Volleyball male players. Main aim of the study was the comparison between Basketball and Volleyball players with different physiological variables. The purpose of the study was to compare resting heart rate, blood pressure (systolic and diastolic), maximum oxygen consumption, anaerobic power, positive breath holding and negative breath holding Basketball and Volleyball players. In present study investigator has taken a total of 16 players (08 Basketball and 08 Volleyball). For interpretation of data a comparative analysis of the selected variable, the‘t’ test was applied. The data of both groups were collected separately for both the variable. Statistic such as mean and standard deviation was computed. The level is significance was set at 0.05. It was found the Basketball and Volleyball players do not have significant deference between the variables: resting heart rate, blood pressure (systolic and diastolic), maximum oxygen consumption, anaerobic power, positive breath holding and negative breath holding.