The purpose of the study was to describe and quantify the changes in blood glucose concentrations with the progression of a simulated volleyball match in the university level volleyball players. Twelve highly trained male volleyball players (age 21.3 +/- 3.0, mean +/- SD) from Viswa-Bharati University, West Bengal, India were selected as subjects, and divided into two team. The selected dependent variable: Blood glucose was assessed by performing 3 simulated sets of volleyball match. Both the teams were balanced in the sense of skill and performance. Data as blood sample were collected at the beginning of the match that is prior to the warm-up, after completion of first set, second set and after third set of the match. Blood samples were taken by pricking the finger. Accu-Chek blood glucose monitor was used to take the reading of blood glucose in the unit mg/dl. Results of current study revealed that there is significant change in the concentration of blood glucose with the progression of a simulated volleyball match. Blood glucose concentration increases from pre set value to post set-2 and decreases after post set-3.( pre set-105.58 +/- 10.130, mean +/- SD ), (post set-1, 119.75+/-12.17) ,( post set-2, 129.25+/-8.72 ), ( post set-3, 123.42+/-10.07). From the results of this study it revealed that there is a significant change in the concentration of blood glucose with the progression of a simulated volleyball match.